Using Autosampler in the Chromatography Laboratory

Autosamplers may be classified by their capacity, such as autosamplers as opposed to auto-injectors; the latter instrument is capable of running more than one sample at once. Robotic instruments offer another classification of autosampler, with rotating/SCARA robots being among the most widely used.

In gas-liquid chromatography, the column inlet (or injector) provides for the introduction of samples into a continuous flow of carrier gas. Common inlet types are the split/splitless injectors, on-column inlets, PTV injectors, the gas source inlet (also called a gas switching valve), purge and trap systems and SPME (solid phase micro extraction) systems. In the split/splitless injector, the sample is introduced to a heated chamber using a syringe.

With an on-column inlet, the sample is introduced in its entirety without the use of heat. PTV injectors introduce the sample through a heated liner at a controlled rate. In the gas source inlet method, the sample is inserted into the gas stream from collection bottles, a method which allows samples to be introduced without interrupting the carrier gas flow.

Purge and trap autosampler systems involve bubbling an inert gas through aqueous samples, purging insoluble volatile compounds from the matrix. These volatile compounds are then trapped in an absorbent column which is then heated – the volatiles are directed into the carrier stream. Solid phase micro extraction (SPME)is a more economical alternative to purge and trap systems which provides greater ease of use and a lower cost.

The type of automatic sampling system used depends largely on the specific application; in gas chromatography alone, there are two different types of columns used – with the different types of autosampler being more appropriate for one or the other. There are packed columns (usually made of glass or stainless steel and containing an inert, solid and highly granular material which is coated with a liquid or solid stationary phase).

The other type are capillary columns; these columns feature a very small internal diameter, with the inside of the column being coated with the phase. Other capillary columns are made with a semi-solid construction and parallel micropores; this style allows for great flexibility, so a long column can be wound into a tight coil which takes up far less room.

While it is gas and liquid chromatography which often first come to mind when discussing different types of autosampler, there are samplers used in many different applications from the life sciences to geological surveys, the pharmaceutical industry, water quality testing and nearly every other application in the materials and life sciences as well as quality control testing of all types. These instruments allow laboratories to handle higher sample throughputs while increasing reproducibility and efficiency.

What is the Alternative Science ?

May science explain everything? Well, that depends on what type of science you are talking about. Sometimes strange occurrences can’t be explained by traditional science. When this happens, alternative science steps in to find a solution for a strange problem. Alternative science has gained a negative reputation throughout the years, but this type of science is also largely misunderstood. Taking a few moments to read about alternative science is a great way to understand how this type of science works. You’ll find that alternative science does not cover traditional scientific matters. Instead, this type of science deals with anomalies and things that are not explained by traditional science.

You can be looking for a number of different things online if you study alternative science. One of these things may include references and research that can support your findings or claims. You will find a lot of this material online. You will also discover that we respect alternative science. We respect this type of science so much that we’ve gone out of our way to collect data and information about various alternative sciences. When we have enough information, we post these details on our Anomalies and Alternative Science Directory page. Our goal is to add enough information to this page, so that you have a fast and easy way to search for anything related to alternative science. If you’re curious about alternative science, begin your search today.

The Effect of Climate Change

The different possible ways of how climate change might affect the world range from the major issues such as extreme weather events, infrastructure failure, etc. But whether climate change is a matter of cold scientific calculation or a moral issue for our generation, its effects are increasingly being felt and observed.

A warming planet can hope to have perceptible disruptions on several aspects, primarily in agriculture. Changes in average global average temperature and atmospheric conditions will cause decline in some crops while causing an increase in others. In some areas like California’s Central Valley, most crops will likely suffer a sharp drop in yields due to factors like decreased bee pollination and shorter cold seasons. Climate change will also affect not only food production but food processing industries as well due to water and energy issues.

Climate change will also accelerate the loop of emissions that has rising temperatures growing populations and energy demands locked in a vicious cycle. Changes in precipitation levels and extreme weather events will also impact power generation systems in many areas, increasing demand for energy that might lead to even greater and more dangerous dependence on fossil fuels.

Outdated infrastructure built to deal with the last few centuries’ climate patterns will have a hard time keeping up or even standing in the face of today’s changing climate. Transportation routes for food, water, fuel and other necessary commodities can be disrupted by infrastructure failure. Extreme weather events that pounded many parts of the globe last year exposed infrastructure vulnerabilities and have alarmed authorities to the possibility of more similar episodes in the future.

The Importance of Energy

Energy is very important in our life.Matter has the capacity to do wok under certain conditions even though it may not be accomplished at a particular moment. A moving of water or water stored behind a dam, for example can be made of work in a variety of at a particular .Gas or air compressed in a tank can also do work when it is released at a particular time. The capacity of matter to do work is generally called energy.


All life on earth depends in some way upon energy. Most of the earth energy comes from the sun. It travels to the earth in rays or waves. Energy in this form is used by plants to make food. Plant food is needed to sustain life by providing nourishment for our bodies and muscles. The sun’s energy is also stored in coal, wood and oil which is also used to produce food and modify matter. It has been estimate that the amount of energy falling on the earth surface annually is equal to the energy of 250 million tons of coal.


Energy is known to exit in a verity of different forms, Among these are heat, light, chemical, electrical, sound, mechanical and nuclear. AS a general rule, energy changes rather easily from on form to another. The steam driven turbine generator of power plant is designed to convert heat energy into electrical energy. The heating system of a building operates by converting the chemical energy of gas or fuel into a usable form of heat. Electrical energy may also be converted into light, heat or mechanical energy though the use of different equipment. The capacity of energy to do work make all of this possible.